Dr. J. Brooks Crozier

Department: Biology
Office: 401A Life Science
Phone: 375-2548



B.S., Roanoke College; M.S., Ph.D., Virginia Polytechnic Institute & State University. 1999

Course List

Research & Teaching Interests:

Microbiology, Mycology (fungi), Phycology (algae), INQ 250(about the microbiology and ecology of soil), INQ 300 (gives students an opportunity to learn about learning and solve a problem in the sciences).

Scholarly Activities:

Microbial Source Tracking, including detection of virulence and host-specific DNA markers; performance criteria for such markers; and their application in target-oriented water resource management.

Currently on Sabbatical (Spring 2013)working on several projects at Virginia Tech and will spend April, May and part of June 2013 in the lab of Dr. Andreas Farnleitner in Vienna Austria.

Recent Publications:

J. B. Crozier and M. Alvarez. 2011. Chapter 27. Training Future Scientists: Teaching Microbial Source Tracking (MST) to Undergraduates in Microbial Source Tracking: Methods, Applications, and Case Studies. Springer publishers.

J. W. Dickerson Jr., J. B. Crozier, C. Hagedorn, and A. Hassall. 2007. Assessment of the 16S-23S rDNA intergenic spacer region in Enterococcus spp. for Microbial Source Tracking. Journal of Environmental Quality. 36:1661-1669.

C. Hagedorn, J.B. Crozier, K.A. Mentz, A.M. Booth, A.K. Graves, N.J Nelson, and R.B. Reneau, Jr. 2003. Carbon Source utilization profiles as a method to identify sources of fecal pollution in water. Journal of Applied Microbiology 94 (5), 792-799.

J. B. Crozier. 2003. Development of a Bacterial Source Tracking Program at a Small Liberal Arts College. Environmental Detection News (4) 1-5.

J.B. Crozier, B. Clark, and H. Weber. 2002. Identifying Sources of Fecal Pollution in the Roanoke River, Roanoke County, Virginia. Virginia Journal of Science, 53:157-165.

Abstracts, poster presentations and other:
J. B. Crozier, M. Mizelle, S. Thomsen, A. Hassell Lawrence, and C. Hagedorn. The qPCR Detectability of a Bovine-Associated Genetic Marker in Composite Fecal Mixtures and in Microcosms. Submitted for consideration (ASM 2012 General meeting).

A. Hassall Lawrence, C. Hagedorn, J. Crozier, M. Saluta, R. Farris. 2012. Determining Sources of Fecal Pollution with qPCR-Bacteroidales in a Uniquely Biodiverse but Threatened Watershed. Submitted for consideration (ASM 2012 General meeting).

Scott, K, and J. Brooks Crozier. 2010. Duration of Detection of Human Bacteroidales Compared to Culturable Enterococcus in Water and sediment. Virginia Branch of the American Society for Microbiology, Lynchburg VA. Poster presented and abstract.

Hogan, L. and J. Brooks Crozier. 2010. Identification of Fungi on Treated Pine Roots by PCR Amplification and Sequencing of the Fungal ITS Region. Virginia Branch of the American Society for Microbiology, Lynchburg VA. Poster presented and Abstract.

J. B. Crozier and K. Scott. Bacteroides: DNA Extraction, Amplification, and Visualization. June 2009. Protocol written and presented day-long workshop for the Virginia Tech Hagedorn lab graduate student training workshop on PCR amplification of human-specific fecal markers. Workshop.

J. B. Crozier, J. Watkinson and K. Filer. 2010. Student Learning in an Undergraduate Non-Majors Soil Microbiology Course in a New Undergraduate General Education Curriculum. JMBE. 11:74-75. Poster presented and Abstract.

J. B. Crozier. Microbial Source Tracking. 2006. IN Pathogen Research Symposium: Pathways and Monitoring in Natural and Engineered Systems, Virginia Water Resources Research Center Special Report.

Hagedorn, C., Touchton, G., Hassall, A., and B. Crozier. 2006. Approaches to Source Classification of Fecal Bacterial Isolates from Water Samples to Improve Results from Library-Dependent Methods. ASM General Meeting, Orlando FL. Poster presented and abstract.